COX-2和bFGF在宫颈癌中的表达及其临床意义

罗春芳; 王晖; 洪慧莉; 芦玉兰; 刘铭球

doi:10.3969/j.issn.1000-8179.2011.07.006

摘要: 目的：研究环氧合酶-2 （COX-2）和碱性成纤维细胞生长因子（bFGF）在宫颈癌组织中的表达相关性及其临床意义。方法：采用组织芯片和免疫组化技术检测143例宫颈浸润癌（ICC）、 20例癌旁正常宫颈上皮（NCE）中COX-2和bFGF的表达，统计分析COX-2、 bFGF表达与病理分级、临床分期和淋巴结转移等临床病理因素的关系。结果： COX-2在ICC和NCE中的阳性表达率分别为87.4% （125/143）和40.0% （8/20）， P<0.001。bFGF在ICC和NCE中的阳性表达率分别为88.8% （127/143）和25.0% （5/20），P<0.001。COX-2的表达与临床分期（r=0.222， P=0.008）、病理分级（r=0.354， P<0.001）和间质浸润深度（r=0.243， P=0.003）呈正相关。bFGF表达分别与淋巴结转移（r=0.239， P=0.004）和病理分级（r=0.369， P<0.001）呈正相关。COX-2和bFGF在宫颈癌组织中的表达强度呈正相关（r=0.234， P=0.005），二者表达一致的比例高达79.7% （114/143）。随着宫颈癌恶性度增高， COX-2和bFGF的表达呈上升趋势。结论： COX-2和bFGF的异常表达与宫颈癌的分化、侵袭和进展有关。联合检测COX-2和bFGF有助于判断宫颈癌的生物学行为和评估患者预后。

Abstract: Expression of COX-2 and bFGF in Cervical Carcinoma and Its Clinical SignificanceChunfang LUO1, HuiWANG2, Huili HONG1, Yulan LU3, Mingqiu LIU4Correspondence to: Chunfang LUO, E-mail: CHFL2001@hotmail.com1Department of Obstetrics and Gynecology,Wuhan No. 3 Hospital,Wuhan 430060, China2Department of Pathology,Wuhan No. 3 Hospital,Wuhan 430060, China3Department of Gynecologic Oncology, Zhongnan Hospital ofWuhan University,Wuhan 430072, China4Department of Pathology and Pathophysiology,Wuhan University School of Medicine,Wuhan 430072, ChinaThis work was supported by Funds of Research Projects fromWuhan Municipal Bureau of Public Health (No.2007-43)Abstract Objective: To investigate the expression of cyclooxygenase-2 ( COX-2 ) and basic fibroblast growth factor ( bFGF )in cervical carcinoma and its clinical significance. Methods: Tissue microarray technique and immunohistochemistry SP method wereused to detect the expression of COX-2 and bFGF in 143 tissue samples of invasive carcinoma of the cervix ( ICC ) and 20 tissue sam-ples of paraneoplastic normal cervical epithelium ( NCE ). The relationship of COX-2 and bFGF expression in ICC with the clinicopath-ologic factors including histopathological grading, nodal metastasis, stroma involvement, FIGO staging and histological type was ana-lyzed. Results: The positive expression of COX-2 was significantly higher in ICC than in NCE 87.4% ( 125/143 ) vs. 40.0% ( 8/20 ) ,P = 0.00 . The positive expression of bFGF was significantly higher in ICC than in NCE 88.8% (127/143) vs. 25.0% (5/20), P =0.00 . The expression of COX-2 was positively correlated with the FIGO staging, histopathological grading and stromal invasion ( r =0.222, P = 0.008; r = 0.354, P = 0.00, and r = 0.243, P = 0.003, respectively ). The expression of bFGF was positively correlated withlymph node metastasis and histopathologic grading ( r = 0.239, P = 0.004 and r = 0.369, P = 0.00, respectively ). The expression of bF-GF was positively correlated with the expression of COX-2 in ICC ( r = 0.234, P = 0.005 ). The ratio of the consistency between the ex-pression of the two proteins was up to 79.7% ( 114/143 ). With the increase of the malignant degree of cervical carcinoma, the expres-sion of COX-2 and bFGF showed a rising tendency. Conclusion: The over-expression of COX-2 and bFGF is closely correlated withthe invasion, progression and differentiation of cervical carcinoma. Joint detection of COX-2 and bFGF expression can help further un-derstand the biological behavior of cervical carcinoma and predict its prognosis.Keywords Cervical carcinoma; Cyclooxygenase-2; Basic fibroblast growth factor; Tissue microarray; Correlation